import win32com.client

wbk = 'C:\\myfolder\\myfile.xlsx'

xlApp = win32com.client.Dispatch("Excel.Application")
xlApp.Workbooks.Open(wbk)

xlAscending = 1
xlSortColumns = 1
xlApp.Sheets(1).Range("A2:C9").Sort(Key1=xlApp.Sheets(1).Range("C1"), Order1=xlAscending, Orientation=xlSortColumns)

xlApp.Quit
xlApp = None